Pathology of infectious diseases

Dr. Tsutsumi's site

Pathology of Infectious Disease

classification of cell

• renewing cell
• stabilized cell
  • proliferate only in a certain condition
• static cell
  • never proliferate

specimens in pathology

• paraffin fixation
  • 3-4 µm thin
• cryostat
• cytology
• electron microscopy
  • 0.1 µm thin

preparation

• fixation by formalin
  • formalin = 37% formaldehyde solution
• delipidation by acetone
• making block in paraffin
• cut by microtome

environmental consideration

• recycle of acetone, xylene
• neutralize formalin

cytology specimen

• desquamatory cytology
• scraping -
• aspiration -
• fine needle aspiration -
• stamp -

fixation in cytology

• Papanicololaou stain - wet fixation with ethanol
  • cells relatively keep their form and size
• Giemsa stain - dry fixation with methanol
  • cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation

staining

hematoxylin eosine

• hematoxylin = blue = for DNA/RNA, nucleus
• eosin = red = for proteins

pathology of infectious diseases

• extracellular pathogens induce neutrophil-based immune reactions
• intracellular pathogens induce macrophage-based cellular immunity