「Pathology of infectious diseases」の版間の差分
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*abscess is defined as aggregation of neutrophils | *abscess is defined as aggregation of neutrophils | ||
*granuloma is defined as aggregation of macrophage and/or T-cells | *granuloma is defined as aggregation of macrophage and/or T-cells | ||
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| + | ==pathology in emerging infectious diseases== | ||
| + | [[File;Pathology_in_emergency_diseease.jpg]] | ||
==病草紙== | ==病草紙== | ||
2021年5月25日 (火) 09:00時点における版
目次
Dr. Tsutsumi's site
Pathology of Infectious Disease
classification of cell
- renewing cell
- stabilized cell
- proliferate only in a certain condition
- static cell
- never proliferate
specimens in pathology
- paraffin fixation
- 3-4 µm thin
- cryostat
- cytology
- electron microscopy
- 0.1 µm thin
preparation
- fixation by formalin
- formalin = 37% formaldehyde solution
- delipidation by acetone
- embedding in paraffin
- cut by microtome
environmental consideration
- recycle of acetone, xylene
- neutralize formalin
cytology specimen
- desquamatory cytology
- scraping -
- aspiration -
- fine needle aspiration -
- stamp -
fixation in cytology
- Papanicololaou stain - wet fixation with ethanol
- cells relatively keep their form and size
- Giemsa stain - dry fixation with methanol
- cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation
staining
hematoxylin eosine
- hematoxylin = blue = for DNA/RNA, nucleus
- eosin = red = for proteins
pathology of infectious diseases
- extracellular pathogens induce neutrophil-based immune reactions
- intracellular pathogens induce macrophage-based cellular immunity
abscess and granuloma
- abscess is defined as aggregation of neutrophils
- granuloma is defined as aggregation of macrophage and/or T-cells
pathology in emerging infectious diseases
File;Pathology_in_emergency_diseease.jpg
