「Scrub typhus」の版間の差分
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Vaccipedia.admin (トーク | 投稿記録) (ページの作成:「==non-malarial fever== *in Asia **dengue **leptospirosis **scrub typhus **typhoid **JEV **influenza **still 30-50% undifferentiated/undiagnosed」) |
Vaccipedia.admin (トーク | 投稿記録) |
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| 3行目: | 3行目: | ||
**dengue | **dengue | ||
**leptospirosis | **leptospirosis | ||
| − | **scrub typhus | + | **'''scrub typhus''' |
**typhoid | **typhoid | ||
**JEV | **JEV | ||
**influenza | **influenza | ||
**still 30-50% undifferentiated/undiagnosed | **still 30-50% undifferentiated/undiagnosed | ||
| + | |||
| + | ==diagnosis/test== | ||
| + | *reason of difficulty of lab test | ||
| + | **obligate intracellular organism | ||
| + | **very diverse stains (i.e., antigens) of ''Orientia'' | ||
| + | **limited lab resources and experties | ||
| + | |||
| + | ===Indirect immunofluorescent assay (IFA)=== | ||
| + | *''Orientia'' antigen - anti-''Orientia'' antibody (primary) - anti- antibody-antigen complex (secondary) labelled by fluorescent | ||
| + | *serial dilution | ||
| + | *microscopic observation | ||
| + | *uses 3 or more primary antibodies (primary antibodies against antigens derived from 3 or more stains) | ||
| + | **genotypes: Karp_A, Karp_B, Saitama, Gilliam, Kato, .... | ||
| + | |||
| + | ===ELISA=== | ||
| + | *''Orientia'' antigen - anti-''Orientia'' antibody (primary) - anti- antibody-antigen complex (secondary) labelled by fluorescent | ||
| + | *serial dilution | ||
| + | *machinery measurement of luminescence | ||
2021年4月15日 (木) 11:05時点における版
non-malarial fever
- in Asia
- dengue
- leptospirosis
- scrub typhus
- typhoid
- JEV
- influenza
- still 30-50% undifferentiated/undiagnosed
diagnosis/test
- reason of difficulty of lab test
- obligate intracellular organism
- very diverse stains (i.e., antigens) of Orientia
- limited lab resources and experties
Indirect immunofluorescent assay (IFA)
- Orientia antigen - anti-Orientia antibody (primary) - anti- antibody-antigen complex (secondary) labelled by fluorescent
- serial dilution
- microscopic observation
- uses 3 or more primary antibodies (primary antibodies against antigens derived from 3 or more stains)
- genotypes: Karp_A, Karp_B, Saitama, Gilliam, Kato, ....
ELISA
- Orientia antigen - anti-Orientia antibody (primary) - anti- antibody-antigen complex (secondary) labelled by fluorescent
- serial dilution
- machinery measurement of luminescence
