「Pathology of infectious diseases」の版間の差分

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*extracellular pathogens induce neutrophil-based immune reactions
 
*extracellular pathogens induce neutrophil-based immune reactions
 
*intracellular pathogens induce macrophage-based cellular immunity
 
*intracellular pathogens induce macrophage-based cellular immunity
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===abscess and granuloma===
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*abscess is defined as aggregation of neutrophils
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*granuloma is defined as aggregation of macrophage and/or T-cells
  
 
==病草紙==
 
==病草紙==

2021年5月24日 (月) 11:57時点における版

Dr. Tsutsumi's site

Pathology of Infectious Disease

classification of cell

  • renewing cell
  • stabilized cell
    • proliferate only in a certain condition
  • static cell
    • never proliferate

specimens in pathology

  • paraffin fixation
    • 3-4 µm thin
  • cryostat
  • cytology
  • electron microscopy
    • 0.1 µm thin

preparation

  • fixation by formalin
    • formalin = 37% formaldehyde solution
  • delipidation by acetone
  • embedding in paraffin
  • cut by microtome

environmental consideration

  • recycle of acetone, xylene
  • neutralize formalin

cytology specimen

  • desquamatory cytology
  • scraping -
  • aspiration -
  • fine needle aspiration -
  • stamp -

fixation in cytology

  • Papanicololaou stain - wet fixation with ethanol
    • cells relatively keep their form and size
  • Giemsa stain - dry fixation with methanol
    • cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation

staining

hematoxylin eosine

  • hematoxylin = blue = for DNA/RNA, nucleus
  • eosin = red = for proteins

pathology of infectious diseases

  • extracellular pathogens induce neutrophil-based immune reactions
  • intracellular pathogens induce macrophage-based cellular immunity

abscess and granuloma

  • abscess is defined as aggregation of neutrophils
  • granuloma is defined as aggregation of macrophage and/or T-cells

病草紙