「Pathology of infectious diseases」の版間の差分
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*Giemsa stain - dry fixation with methanol | *Giemsa stain - dry fixation with methanol | ||
**cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation | **cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation | ||
| + | |||
| + | ==staining== | ||
| + | ===hematoxylin eosine=== | ||
| + | *hematoxylin = blue = for DNA/RNA, nucleus | ||
| + | *eosin = red = for proteins | ||
2021年5月24日 (月) 09:59時点における版
目次
classification of cell
- renewing cell
- stabilized cell
- proliferate only in a certain condition
- static cell
- never proliferate
specimens in pathology
- paraffin fixation
- 3-4 µm thin
- cryostat
- cytology
- electron microscopy
- 0.1 µm thin
preparation
- fixation by formalin
- formalin = 37% formaldehyde solution
- delipidation by acetone
- making block in paraffin
- cut by microtome
environmental consideration
- recycle of acetone, xylene
- neutralize formalin
cytology specimen
- desquamatory cytology
- scraping -
- aspiration -
- fine needle aspiration -
- stamp -
fixation in cytology
- Papanicololaou stain - wet fixation with ethanol
- cells relatively keep their form and size
- Giemsa stain - dry fixation with methanol
- cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation
staining
hematoxylin eosine
- hematoxylin = blue = for DNA/RNA, nucleus
- eosin = red = for proteins
