Pathology of infectious diseases
目次
Dr. Tsutsumi's site
Pathology of Infectious Disease
classification of cell
- renewing cell
- stabilized cell
- proliferate only in a certain condition
- static cell
- never proliferate
specimens in pathology
- paraffin fixation
- 3-4 µm thin
- cryostat
- cytology
- electron microscopy
- 0.1 µm thin
preparation
- fixation by formalin
- formalin = 37% formaldehyde solution
- delipidation by acetone
- embedding in paraffin
- cut by microtome
environmental consideration
- recycle of acetone, xylene
- neutralize formalin
cytology specimen
- desquamatory cytology
- scraping -
- aspiration -
- fine needle aspiration -
- stamp -
fixation in cytology
- Papanicololaou stain - wet fixation with ethanol
- cells relatively keep their form and size
- Giemsa stain - dry fixation with methanol
- cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation
staining
hematoxylin eosine
- hematoxylin = blue = for DNA/RNA, nucleus
- eosin = red = for proteins
pathology of infectious diseases
- extracellular pathogens induce neutrophil-based immune reactions
- intracellular pathogens induce macrophage-based cellular immunity
abscess and granuloma
- abscess is defined as aggregation of neutrophils
- granuloma is defined as aggregation of macrophage and/or T-cells
