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*Outer membrane catches crystal violet with iodine but easily lose them by ethanol rinsing
[[File:Gram_Stain.png|none|800px]]
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{{collapse
|title=
'''Classify major Gram-Positive cocci'''
|content=
There are so many species of gram-positive cocci.
*Catalase positive in tubes
**''Staphylococcus'' spp.
***Coagulase positive in tubes
****''Staphylococcus aureus''
***Coagulase negative in tubes
****''Staphylococcus epidermidis''
****''Staphylococcus saprophyticus''
*Catalase negative in tubes
**''Streptococcus'' spp.
***Alpha-hemolytic (partial hemolysis with green color)
****''Streptococcus pneumoniae''
****''Streptococcus suis''
****viridans ''Streptococcus''
***Beta-hemolytic (complete hemolysis)
****Group A (beta) streptococci (''Streptococcus pyogenes'')
****Group B (beta) streptococci (''Streptococcus agalactae'')
****Group C (beta) streptococci
****Group G (beta) streptococci
***Gamma-hemolytic (no hemolysis)
****''Streptococcus bovis''
****''Enterococcus faecium''
****''Enterococcus faecalis''
}}
{{collapse
|title=
'''Classify major Gram-Positive bacilli'''
|content=
Smaller number of bacilli show gram-positive.
*Spore forming
**Aerobic
***''Bacillus anthracis''
***''Bacillus cereus''
**Anaerobic
***''Clostridium tetani''
***''Clostridium perfringens''
*Non-spore forming
**Aerobic
***''Corynebacterium diphtheria''
**Anaerobic
***''Listeria monocytogenes''
}}
