**Genome detection by PCR and other NAATs (nucleic acid amplification tests)
**Antigen detection by ELISA and immunochromatography, etc.
**Virus isolation using human/animal cells
*Detection of antibody
**IgM or IgG by ELISA and immunochromatography, etc.
**Neutralizing antibody using human/animal cells
}}
{{collapse
|title=
'''Definition of arboviruses'''
|content=
}}
{{collapse
|title=
'''Classification of major arboviruses and their major vectors'''
|content=
}}
{{collapse
|title=
'''Warning signs of severe dengue'''
|content=
}}
{{collapse
|title=
'''List of Vaccine-preventable arbovirus infections'''
|content=
}}
{{collapse
|title=
'''Essential knowledge of HIV'''
|content=
[https://drive.google.com/file/d/1N5GBSimejHLEk9We-fEC240mgjuT5-PC/view?usp=share_link Watch our Group 4 video and pick up information described in blue letters!]
}}
==Bacteria==
{{collapse
|title=
'''Biological basis of Gram staining'''
|content=
*Bacteria have both of '''cell wall''' and '''cell membrane'''
**Human and other animal cells have no cell wall
**Plant cells have cell wall
*Some bacteria have thick cell wall without outer membrane
*Other bacteria have thin cell wall with outer membrane
*Cell wall contains a layer of Peptidoglycan
[[File:File_Bacterial_cell_walls.jpg|none|400px]]
*Thick peptidoglycan layer catches and keep crystal violet with iodine
*Outer membrane catches crystal violet with iodine but easily lose them by ethanol rinsing
*Bacterial structure different from other major bacteria
**Too thick lipid-rich cell wall; acid fast
*Epidemiology and disease burden
*Three species causing clinical tuberculosis
**''Mycobacterium tuberculosis''
**''M. bovis''
**''M. africanum'' (only in Africa)
*Airborne transmission
**No contact/fomite transmission
**Contaminated milk ingestion may cause food-borne tuberculosis by ''M. bovis'' (''bovis'' means cow)
*Three major Symptoms
**Cough for weeks
**Weight loss for weeks
**Night sweats for weeks
*Diagnostic methods
**sputum microscopy (Ziel-Nielsen staining)
**genome detection by GeneXpert (also detects resistance)
**culture (6-8 weeks)
**chest X-ray
**urine LAM assay
**Mantoux test and IGRA for latent infection
*Potential of long-term (months, years to decades) infection
**Human immune system cannot eliminate established infection but only contains (just to 'hide' ''Mycobacterium'')
*HIV-Tb co-infection is soooooo major public health problem
*Treatment
**6 months (2 intensive + 4 maintenance)
*Definition of drug resistance
**MDR
**XDR
}}
==Protozoa==
{{collapse
|title=
'''How protozoa differ from bacteria'''
|content=
}}
{{collapse
|title=
'''Classification of major protozoa in terms of reproduction manners and infecting organs/cells'''
|content=
-> [[Overview_of_protozoa]]
}}
{{collapse
|title=
'''Essential knowledge of malaria'''
|content=
*Epidemiology and disease burden of malaria worldwide
**Especially of ''Plasmodium falciparum''
*Essential lifecycle
#Mosquito stage
#Human liver stage
#Human red cell stage
*Only ''P. vivax'' and ''P. ovale'' have hypnotic stage in liver cause '''relapse'''
**All species cause '''recrudescence''' due to inadequate/improper treatment
*Fever, Anemia, Splenomegaly
*Cerebral malaria almost only by ''P. falciparum'', which leads to high mortality
*Diagnosis made by Rapid Diagnostic Test (RDT) and Blood smear microscopy (Giemsa staining)
**Blood smear includes thick smear (just to detect ''Plasmodium'') and thin smear (confirm species of ''Plasmodium'' and degree of parasitemia as disease severity)
}}
==Parasite==
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|title=
'''Major classification of parasites'''
|content=
*Helminth
**Nematode (round worm)
**Trematode (fluke, distoma)
**Cestode (tape worm)
*Ectoparasite
}}
==Zoonosis==
{{collapse
|title=
'''Definition of Zoonosis'''
|content=
}}
{{collapse
|title=
'''List of major zoonoses and their host animals'''