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*duration of activity in environment/sewage- seeding period of years means duration of activity of each virus or continuous producing of VDPV due to OPV *how to keep activity in environment/sewage- can the virus propagate in some cells in sewage*any intervention directly to sewage where VDPV has been found, like disinfectant
*where the sabin virus obtains mutation to VDPV
*threshold of immunization coverage to maintain VDPV free
**⇔Variola cases and true smallpox infection overlapped almost identically
*novel OPV2
**using genetically modified stain
**more stabilized genomically
==clinical==
