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==non-malarial fever==
*in Asia
**dengue
**leptospirosis
**'''scrub typhus'''
**typhoid
**JEV
**influenza
**still 30-50% undifferentiated/undiagnosed
==diagnosis/test==
*reason of difficulty of lab test
**obligate intracellular organism
**very diverse stains (i.e., antigens) of ''Orientia''
**limited lab resources and experties
===Indirect immunofluorescent assay (IFA)===
*''Orientia'' antigen - anti-''Orientia'' antibody (primary) - anti- antibody-antigen complex (secondary) labelled by fluorescent
*serial dilution
*microscopic observation
*uses 3 or more primary antibodies (primary antibodies against antigens derived from 3 or more stains)
**genotypes: Karp_A, Karp_B, Saitama, Gilliam, Kato, ....
===ELISA===
*''Orientia'' antigen - anti-''Orientia'' antibody (primary) - anti- antibody-antigen complex (secondary) labelled by fluorescent
*serial dilution
*machinery measurement of luminescence
