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==Dr. Tsutsumi's site==
 
[https://pathos223.com/en/ Pathology of Infectious Disease]
 
[https://pathos223.com/en/ Pathology of Infectious Disease]
  
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**formalin = 37% formaldehyde solution
 
**formalin = 37% formaldehyde solution
 
*delipidation by acetone
 
*delipidation by acetone
*making block in paraffin
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*embedding in paraffin
 
*cut by microtome
 
*cut by microtome
  
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*hematoxylin = blue = for DNA/RNA, nucleus
 
*hematoxylin = blue = for DNA/RNA, nucleus
 
*eosin = red = for proteins
 
*eosin = red = for proteins
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==pathology of infectious diseases==
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*extracellular pathogens induce neutrophil-based immune reactions
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*intracellular pathogens induce macrophage-based cellular immunity
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===abscess and granuloma===
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*abscess is defined as aggregation of neutrophils
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*granuloma is defined as aggregation of macrophage and/or T-cells
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==pathology in emerging infectious diseases==
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[[File:Pathology_in_emergency_diseease.jpg|500px]]
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==病草紙==
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*[https://www.kyohaku.go.jp/jp/syuzou/meihin/emaki/item04.html 病草紙|京都国立博物館]
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*[https://ja.wikipedia.org/wiki/%E7%97%85%E8%8D%89%E7%B4%99 Wikipedia]
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*[https://artsandculture.google.com/exhibit/zAIysMohoOz3Jg?hl=ja Google Arts&Culture]

2021年5月30日 (日) 17:43時点における最新版

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Dr. Tsutsumi's site

Pathology of Infectious Disease

classification of cell

  • renewing cell
  • stabilized cell
    • proliferate only in a certain condition
  • static cell
    • never proliferate

specimens in pathology

  • paraffin fixation
    • 3-4 µm thin
  • cryostat
  • cytology
  • electron microscopy
    • 0.1 µm thin

preparation

  • fixation by formalin
    • formalin = 37% formaldehyde solution
  • delipidation by acetone
  • embedding in paraffin
  • cut by microtome

environmental consideration

  • recycle of acetone, xylene
  • neutralize formalin

cytology specimen

  • desquamatory cytology
  • scraping -
  • aspiration -
  • fine needle aspiration -
  • stamp -

fixation in cytology

  • Papanicololaou stain - wet fixation with ethanol
    • cells relatively keep their form and size
  • Giemsa stain - dry fixation with methanol
    • cells are elongated on slide and appeared size is multiplied in 3-4 times than wet fixation

staining

hematoxylin eosine

  • hematoxylin = blue = for DNA/RNA, nucleus
  • eosin = red = for proteins

pathology of infectious diseases

  • extracellular pathogens induce neutrophil-based immune reactions
  • intracellular pathogens induce macrophage-based cellular immunity

abscess and granuloma

  • abscess is defined as aggregation of neutrophils
  • granuloma is defined as aggregation of macrophage and/or T-cells

pathology in emerging infectious diseases

Pathology in emergency diseease.jpg

病草紙